HAL will be down for maintenance from Friday, June 10 at 4pm through Monday, June 13 at 9am. More information
Skip to Main content Skip to Navigation
Journal articles

Modeling Fixed Bed Bioreactors for Isopropanol and Butanol Production using Clostridium Beijerinckii DSM 6423 Immobilized on Polyurethane Foams

Abstract : Isopropanol-butanol fermentation carried out by C. beijerinckii can be improved by immobilizing cells in a fixed bed bioreactor. In this study, a fixed bed bioreactor was developed using polyurethane foams as a solid support for biofilm growth. The dilution rate was increased over time to follow biofilm development while avoiding solvent accumulation within the bioreactor. A phenomenological model was designed and successfully described batch and continuous fixed bed bioreactor dynamics. The parameters regarding biofilm detachment and attachment were estimated using a bioreactor design allowing biofilm sampling during a continuous fermentation. This last experiment was operated during 550 h and reached a maximum productivity of 1.5 g.L −1.h −1. The model prediction capacity was tested by describing a continuous fermentation in a fixed bed bioreactor column over 800 h. This latter bioreactor enabled a maximum productivity of 2.5 g.L −1 h −1 in 650 h of continuous fermentation. A high solvent concentration (13.5 g.L −1) with a butanol concentration close to the toxicity threshold (7.5 g.L −1) was obtained during the fermentation process. The process enhanced the isopropanol butanol fermentation productivity. The model properly described the batch fermentation and the immobilized cells fermentation. However, a 20% difference between experimental and modeled data was observed. Therefore, the model should be improved to better described such fermentation systems.
Document type :
Journal articles
Complete list of metadata

https://hal-ifp.archives-ouvertes.fr/hal-03602473
Contributor : Catherine Belli Connect in order to contact the contributor
Submitted on : Wednesday, March 9, 2022 - 10:37:53 AM
Last modification on : Saturday, March 12, 2022 - 3:08:56 AM

Identifiers

Collections

IFP

Citation

Maxime Carrié, Hélène Velly, Fadhel Ben-Chaabane, Jean-Christophe Gabelle. Modeling Fixed Bed Bioreactors for Isopropanol and Butanol Production using Clostridium Beijerinckii DSM 6423 Immobilized on Polyurethane Foams. Biochemical Engineering Journal, Elsevier, 2022, 180, pp.108355. ⟨10.1016/j.bej.2022.108355⟩. ⟨hal-03602473⟩

Share

Metrics

Record views

24