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Modeling Fixed Bed Bioreactors for Isopropanol and Butanol Production using Clostridium Beijerinckii DSM 6423 Immobilized on Polyurethane Foams

Abstract : Isopropanol-butanol fermentation carried out by C. beijerinckii can be improved by immobilizing cells in a fixed bed bioreactor. In this study, a fixed bed bioreactor was developed using polyurethane foams as a solid support for biofilm growth. The dilution rate was increased over time to follow biofilm development while avoiding solvent accumulation within the bioreactor. A phenomenological model was designed and successfully described batch and continuous fixed bed bioreactor dynamics. The parameters regarding biofilm detachment and attachment were estimated using a bioreactor design allowing biofilm sampling during a continuous fermentation. This last experiment was operated during 550 h and reached a maximum productivity of 1.5 g.L −1.h −1. The model prediction capacity was tested by describing a continuous fermentation in a fixed bed bioreactor column over 800 h. This latter bioreactor enabled a maximum productivity of 2.5 g.L −1 h −1 in 650 h of continuous fermentation. A high solvent concentration (13.5 g.L −1) with a butanol concentration close to the toxicity threshold (7.5 g.L −1) was obtained during the fermentation process. The process enhanced the isopropanol butanol fermentation productivity. The model properly described the batch fermentation and the immobilized cells fermentation. However, a 20% difference between experimental and modeled data was observed. Therefore, the model should be improved to better described such fermentation systems.
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https://hal-ifp.archives-ouvertes.fr/hal-03602473
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Submitted on : Wednesday, March 9, 2022 - 10:37:53 AM
Last modification on : Saturday, March 12, 2022 - 3:08:56 AM

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Maxime Carrié, Hélène Velly, Fadhel Ben-Chaabane, Jean-Christophe Gabelle. Modeling Fixed Bed Bioreactors for Isopropanol and Butanol Production using Clostridium Beijerinckii DSM 6423 Immobilized on Polyurethane Foams. Biochemical Engineering Journal, Elsevier, 2022, 180, pp.108355. ⟨10.1016/j.bej.2022.108355⟩. ⟨hal-03602473⟩

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