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Gene regulatory network analysis of Trichoderma reesei grown on glucose-lactose mixtures suggests links between development and cellulase production
Abstract : Background: The degradation of cellulose and hemicellulose molecules into
simpler sugars such as glucose is part of the second generation biofuel production
process. Hydrolysis of lignocellulosic substrates is usually performed by enzymes
produced and secreted by the fungus Trichoderma reesei . Studies identifying
transcription factors involved in the regulation of cellulase production have been
conducted but no overview of the whole regulation network is available. A
transcriptomic approach with mixtures of glucose and lactose, used as a substrate
for cellulase induction, was used to help us decipher missing parts in the network.
Results: Experimental results confirmed the impact of sugar mixture on the
enzymatic cocktail composition. The transcriptomic study shows a temporal
regulation of the main transcription factors and a lactose concentration impact
on the transcriptional profile. A gene regulatory network (GRN) built using the
BRANE Cut software reveals three sub-networks related to i) a positive
correlation between lactose concentration and cellulase production, ii) a
particular dependence of the lactose onto the beta-glucosidase regulation and iii) a
negative regulation of the development process and growth.
Conclusions: This work is the first investigating a transcriptomic study regarding
the effects of pure and mixed carbon sources in a fed-batch mode. Our study
expose a co-orchestration of xyr1, clr2 and ace3 for cellulase and hemicellulase
induction and production, a fine regulation of the beta-glucosidase and a decrease of
growth in favor of cellulase production. These conclusions provide us with
potential targets for further genetic engineering leading to better
cellulase-producing strains.
https://hal-ifp.archives-ouvertes.fr/hal-02805426
Contributor : Laurent Duval <>
Submitted on : Saturday, June 6, 2020 - 12:27:54 AM
Last modification on : Wednesday, June 17, 2020 - 3:42:05 PM
Contributor : Laurent Duval <>
Submitted on : Saturday, June 6, 2020 - 12:27:54 AM
Last modification on : Wednesday, June 17, 2020 - 3:42:05 PM
