Origin of Short-Chain Organic Acids in Serpentinite Mud Volcanoes of the Mariana Convergent Margin

Philip Eickenbusch (1) , Ken Takai (2) , Olivier Sissman (3) , Shino Suzuki (4) , Catriona Menzies (5, 6) , Sanae Sakai (2) , Pierre Sansjofre (7) , Eiji Tasumi (2) , Bernasconi Sm (8) , Clemens Glombitza (1, 9) , Bo Barker Jørgensen (10) , Yuki Morono (4) , Mark A Lever (1)

1 IBP - Institute of Biogeochemistry and Pollutant Dynamics [ETH Zürich]
2 JAMSTEC - Japan Agency for Marine-Earth Science and Technology
3 IFPEN - IFP Energies nouvelles
4 Kochi Institute for Core Sample Research
5 NOC - National Oceanography Centre [Southampton]
6 Department of Geology and Petroleum Geology [Aberdeen]
7 LGO - Laboratoire Géosciences Océan
8 Geological Institute [ETH Zürich]
9 ARC - NASA Ames Research Center
10 Aarhus University [Aarhus]

Serpentinitic systems are potential habitats for microbial life due to frequently high concentrations of microbial energy substrates, such as hydrogen (H 2), methane (CH 4), and short-chain organic acids (SCOAs). Yet, many serpentinitic systems are also physiologically challenging environments due to highly alkaline conditions (pH > 10) and elevated temperatures (>80 • C). To elucidate the possibility of microbial life in deep serpentinitic crustal environments, International Ocean Discovery Program (IODP) Expedition 366 drilled into the Yinazao, Fantangisña, and Asùt Tesoru serpentinite mud volcanoes on the Mariana Forearc. These mud volcanoes differ in temperature (80, 150, 250 • C, respectively) of the underlying subducting slab, and in the porewater pH (11.0, 11.2, 12.5, respectively) of the serpentinite mud. Increases in formate and acetate concentrations across the three mud volcanoes, which are positively correlated with temperature in the subducting slab and coincide with strong increases in H 2 concentrations, indicate a serpentinization-related origin. Thermodynamic calculations suggest that formate is produced by equilibrium reactions with dissolved inorganic carbon (DIC) + H 2 , and that equilibration continues during fluid ascent at temperatures below 80 • C. By contrast, the mechanism(s) of acetate production are not clear. Besides formate, acetate, and H 2 data, we present concentrations of other SCOAs, methane, carbon monoxide, and sulfate, δ 13 C-data on bulk carbon pools, and microbial cell counts. Even though calculations indicate a wide range of microbial catabolic reactions to be thermodynamically favorable, concentration profiles of potential energy substrates, and very low cell numbers suggest that microbial life is scarce or absent. We discuss the potential roles of temperature, pH, pressure, and dispersal in limiting the occurrence of microbial life in deep serpentinitic environments.

Mots clés: limits of life; deep biosphere; serpentinization; abiotic synthesis; formate; acetate; methane; International Ocean Discovery Program

Domaines: Sciences de l'environnement; Océan, Atmosphère; Sciences de la Terre

Philip Eickenbusch, Ken Takai, Olivier Sissman, Shino Suzuki, Catriona Menzies, et al.. Origin of Short-Chain Organic Acids in Serpentinite Mud Volcanoes of the Mariana Convergent Margin. Frontiers in Microbiology, 2019, 10, ⟨10.3389/fmicb.2019.01729⟩. ⟨hal-02275213⟩