S. Forcisi, F. Moritz, B. Kanawati, D. Tziotis, R. Lehmann et al., Liquid 477 chromatography-mass spectrometry in metabolomics research: mass analyzers in ultra high 478 pressure liquid chromatography coupling, J. Chromatogr. A, vol.1292, pp.51-65, 2013.

,

P. Paíga, L. M. Silva, and C. Delerue-matos, Optimization of the Ion Source-Mass Spectrometry 481 Parameters in Non-Steroidal Anti-Inflammatory and Analgesic Pharmaceuticals Analysis by a 482 Design of Experiments Approach, J. Am. Soc. Mass Spectrom, vol.27, issue.10, pp.1703-1714, 2016.

A. Grand-guillaume-perrenoud, J. Veuthey, and D. Guillarme, Coupling state-of-the-art 485 supercritical fluid chromatography and mass spectrometry: From hyphenation interface 486 optimization to high-sensitivity analysis of pharmaceutical compounds, J. Chromatogr. A, vol.1339, pp.174-184, 2014.

M. D. Peris-díaz, M. A. Sentandreu, and E. Sentandreu, Multiobjective optimization of liquid 489 chromatography-triple-quadrupole mass spectrometry analysis of underivatized human urinary 490 amino acids through chemometrics, Anal. Bioanal. Chem, vol.410, issue.18, pp.4275-4284, 2018.

,

T. M. Jarrell, C. L. Marcum, H. Sheng, B. C. Owen, C. J. O'lenick et al.,

. Kenttämaa, Characterization of organosolv switchgrass lignin by using high performance liquid 494 chromatography/high resolution tandem mass spectrometry using hydroxide-doped negative-495 ion mode electrospray ionization, Green Chem, vol.16, issue.5, pp.2713-2727, 2014.

E. Kiyota, P. Mazzafera, and A. C. Sawaya, Analysis of soluble lignin in sugarcane by 498 ultrahigh performance liquid chromatography-tandem mass spectrometry with a do-it-yourself 499 oligomer database, Anal. Chem, vol.84, issue.16, pp.7015-7020, 2012.

B. C. Owen, L. J. Haupert, T. M. Jarrell, C. L. Marcum, T. H. Parsell et al.,

H. I. Black and . Kenttämaa, High-performance liquid chromatography/high-resolution multiple stage 502 tandem mass spectrometry using negative-ion-mode hydroxide-doped electrospray ionization 503 for the characterization of lignin degradation products, Anal. Chem, vol.84, issue.14, pp.6000-6007, 2012.

C. Diego, Upgrading of biomass: alternative ways for biomass treatment, p.506, 2014.

P. F. Harmsen, W. J. Huijgen, L. M. Bermudez-lopez, and R. R. Bakker, Literature review of 507 physical and chemical pretreatment processes for lignocellulosic biomass, vol.3, p.508, 2010.

A. V. Bridgwater, Review of fast pyrolysis of biomass and product upgrading, Biomass and 510 Bioenergy, vol.38, pp.68-94, 2012.

D. Meier, B. Van-de-beld, A. V. Bridgwater, D. C. Elliott, A. Oasmaa et al., State-of-the-art of 512 fast pyrolysis in IEA bioenergy member countries, Renewable and Sustainable Energy Reviews, vol.513, issue.20, pp.619-641, 2013.

N. Charon, J. Ponthus, D. Espinat, F. Broust, G. Volle et al., Multi-technique 515 characterization of fast pyrolysis oils, Journal of Analytical and Applied Pyrolysis, vol.116, pp.18-516, 2015.

J. H. Marsman, J. Wildschut, F. Mahfud, and H. J. Heeres, Identification of components in fast 518 pyrolysis oil and upgraded products by comprehensive two-dimensional gas chromatography 519 and flame ionisation detection, J. Chromatogr. A, vol.1150, issue.1-2, pp.21-27, 2007.

,

K. Sipila, E. Kuoppala, L. Fagernas, and A. Oasmaa, CHARACTERIZATION OF BIOMASS-BASED FLASH 522 PYROLYSIS OILS, Biomass and Bioenergy, vol.14, issue.2, pp.103-113, 1998.

M. Sta?, D. Kubi?ka, J. Chudoba, and M. Pospí?il, Overview of Analytical Methods Used for Chemical 524 Characterization of Pyrolysis Bio-oil, Energy Fuels, vol.28, issue.1, pp.385-402, 2013.

A. Le-masle, D. Angot, C. Gouin, A. D'attoma, J. Ponthus et al., Development 527 of on-line comprehensive two-dimensional liquid chromatography method for the separation of 528 biomass compounds, J. Chromatogr. A, vol.1340, pp.90-98, 2014.

,

D. Tomasini, F. Cacciola, F. Rigano, D. Sciarrone, P. Donato et al., , p.531

L. Mondello, Complementary analytical liquid chromatography methods for the characterization 532 of aqueous phase from pyrolysis of lignocellulosic biomasses, Anal. Chem, vol.86, issue.22, pp.11255-533, 2014.

J. Hertzog, V. Carré, Y. L. Brech, A. Dufour, and F. Aubriet, Toward Controlled Ionization Conditions 535 for ESI-FT-ICR-MS Analysis of Bio-Oils from Lignocellulosic Material, Energy Fuels, vol.30, issue.7, pp.536-5729, 2016.

J. Hertzog, V. Carré, Y. L. Brech, C. L. Mackay, A. Dufour et al., Combination of 538 electrospray ionization, atmospheric pressure photoionization and laser desorption ionization 539

, Fourier transform ion cyclotronic resonance mass spectrometry for the investigation of complex 540 mixtures -Application to the petroleomic analysis of bio-oils, Anal. Chim. Acta, vol.969, pp.26-541, 2017.

Y. Liu, Q. Shi, Y. Zhang, Y. He, K. H. Chung et al.,

, Bio-oil by Gas Chromatography-Mass Spectrometry and Negative-Ion Electrospray Ionization 544

, Energy Fuels, vol.26, issue.7, pp.545-4532, 2012.

J. M. Jarvis, A. M. Mckenna, R. N. Hilten, K. C. Das, R. P. Rodgers et al., , p.547

, Pine Pellet and Peanut Hull Pyrolysis Bio-oils by Negative-Ion Electrospray Ionization Fourier 548 Transform Ion Cyclotron Resonance Mass Spectrometry, Energy Fuels, vol.26, issue.6, pp.3810-3815, 2012.

E. A. Smith, S. Park, A. T. Klein, and Y. J. Lee, Bio-oil Analysis Using Negative Electrospray Ionization: 551 Comparative Study of High-Resolution Mass Spectrometers and Phenolic versus Sugaric 552

, Energy Fuels, vol.26, issue.6, pp.3796-3802, 2012.

P. V. Abdelnur, B. G. Vaz, J. D. Rocha, M. B. De-almeida, M. A. Teixeira et al., 554 Characterization of Bio-oils from Different Pyrolysis Process Steps and Biomass Using High-555 Resolution Mass Spectrometry, vol.27, pp.6646-6654, 2013.

,

M. Sta?, J. Chudoba, M. Auersvald, D. Kubi?ka, S. Conrad et al., Application of 558 orbitrap mass spectrometry for analysis of model bio-oil compounds and fast pyrolysis bio-oils 559 from different biomass sources, Journal of Analytical and Applied Pyrolysis, vol.124, pp.230-238, 2017.

K. S. Boes, R. H. Narron, Y. Chen, S. Park, and N. R. Vinueza, Characterization of biofuel refinery 562 byproduct via selective electrospray ionization tandem mass spectrometry, Fuel, vol.188, pp.190-196, 2017.

K. S. Boes, R. H. Narron, S. Park, and N. R. Vinueza, Mass Spectrometry Exposes Undocumented Lignin-565

, Carbohydrate Complexes in Biorefinery Pretreatment Stream, vol.6, pp.10654-10659, 2018.

J. Prothmann, M. Sun, P. Spégel, M. Sandahl, and C. Turner, Ultra-high-performance supercritical 568 fluid chromatography with quadrupole-time-of-flight mass spectrometry (UHPSFC/QTOF-MS) 569 for analysis of lignin-derived monomeric compounds in processed lignin samples, Anal. Bioanal

, Chem, vol.409, issue.30, pp.7049-7061, 2017.

J. Crepier, A. Le-masle, N. Charon, F. Albrieux, P. Duchene et al., Ultra-high performance 572 supercritical fluid chromatography hyphenated to atmospheric pressure chemical ionization 573 high resolution mass spectrometry for the characterization of fast pyrolysis bio-oils, J. 574 Chromatogr. B Analyt. Technol. Biomed. Life Sci, vol.1086, pp.38-46, 2018.

,

N. Lemonakis, A. Skaltsounis, A. Tsarbopoulos, and E. Gikas, Optimization of parameters affecting 577 signal intensity in an LTQ-orbitrap in negative ion mode: A design of experiments approach, p.578

, Talanta, vol.147, pp.402-409, 2016.

M. Dole, L. L. Mack, R. L. Hines, R. C. Mobley, L. D. Ferguson et al., Molecular Beams of, p.580

. Macroions, The Journal of Chemical Physics, vol.49, issue.5, pp.2240-2249, 1968.

J. V. Iribarne and B. A. Thomson, On the evaporation of small ions from charged droplets, The Journal 583 of Chemical Physics, vol.64, p.2287, 1976.

M. Wilm, Principles of electrospray ionization, Mol. Cell. Proteomics, vol.10, issue.7, 2011.

P. Kebarle and M. Peschke, On the mechanisms by which the charged droplets produced by 587 electrospray lead to gas phase ions, Anal. Chim. Acta, vol.406, issue.1, pp.11-35, 2000.

, , p.598

M. Bernardin, F. Bessueille-barbier, A. Le-masle, C. Lienemann, and S. Heinisch, Suitable interface 590 for coupling liquid chromatography to inductively coupled plasma-mass spectrometry for the 591 analysis of organic matrices. 1 Theoretical and experimental considerations on solute 592 dispersion, J. Chromatogr. A, vol.1565, pp.68-80, 2018.

D. Wolrab, P. Frühauf, and C. Gerner, Direct coupling of supercritical fluid chromatography with 594 tandem mass spectrometry for the analysis of amino acids and related compounds: Comparing 595 electrospray ionization and atmospheric pressure chemical ionization, Anal. Chim. Acta, vol.981, pp.106-115, 2017.

J. W. Thompson, J. W. Eschelbach, R. T. Wilburn, and J. W. Jorgenson, Investigation of electrospray 598 ionization and electrostatic focusing devices using a three-dimensional electrospray current 599 density profiler, J. Am. Soc. Mass Spectrom, vol.16, issue.3, pp.312-323, 2005.

,

D. P. Smith, The Electrohydrodynamic Atomization of Liquids, IEEE Trans. on Ind. Applicat. IA-602, vol.22, issue.3, pp.527-535, 1986.

R. Dams, T. Benijts, W. Günther, W. Lambert, and A. De-leenheer, Influence of the eluent 604 composition on the ionization efficiency for morphine of pneumatically assisted electrospray, 605 atmospheric-pressure chemical ionization and sonic spray, Rapid Commun. Mass Spectrom, vol.16, issue.11, pp.1072-1077, 2002.

B. A. Huffman, M. L. Poltash, and C. A. Hughey, Effect of polar protic and polar aprotic solvents on 608 negative-ion electrospray ionization and chromatographic separation of small acidic molecules, Anal. Chem, vol.84, issue.22, pp.9942-9950, 2012.

R. Kostiainen and A. P. Bruins, Effect of Solvent on Dynamic Range and Sensitivity in Pneumatically-611 assisted Electrospray (Ion Spray) Mass Spectrometry, Rapid Commun. Mass Spectrom, vol.10, issue.11, pp.1393-1399, 1996.

R. Kostiainen and T. J. Kauppila, Effect of eluent on the ionization process in liquid chromatography-615 mass spectrometry, J. Chromatogr. A, vol.1216, issue.4, pp.685-699, 2009.

,

P. Kebarle and /. Tang, From ions in solution to ions in the gas phase -the mechanism of 618 electrospray mass spectrometry, Anal. Chem, vol.65, issue.22, pp.972-986, 1993.

Z. Wu, W. Gao, M. A. Phelps, D. Wu, D. D. Miller et al., Favorable effects of weak acids on 620 negative-ion electrospray ionization mass spectrometry, Anal. Chem, vol.76, issue.3, pp.839-847, 2004.

N. B. Cech and C. G. Enke, Practical implications of some recent studies in electrospray ionization 623 fundamentals, Mass Spectrom. Rev, vol.20, issue.6, pp.362-387, 2001.