Enzymatic hydrolysis at high lignocellulosic content: Optimization of the mixing system geometry and of a fed-batch strategy to increase glucose concentration

Abstract : Working at high values of lignocellulosic Dry Matter (DM), as wheat straw, increases the reaction medium viscosity, making the mixing inefficient with the traditional agitators. Batch and fed-batch tests were conducted using different impellers: i) inclined blades, ii) marine impeller, iii) anchor, iv) paravisc and v) double helical impeller. Inclined blades appeared an inadequate device for batch and fed-batch tests. On contrary, double helical impellers and anchor gave optimal performances. An alternative to improve the reactor’s rheology is the modification of the feeding strategy. A particular fed-batch strategy allowed keeping low the reaction medium viscosity by a gradual increasing of the DM content in the reactor. In this way, three main benefits were achieved: i) a very good performances in terms of glucose concentration (85 g/L), ii) a strong reduction of the energetic consumption compared to batch test and iii) the adoption of a simple mixing devise.
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Federico Battista, Mélanie Gomez Almendros, Romain Rousset, Pierre-Antoine Bouillon. Enzymatic hydrolysis at high lignocellulosic content: Optimization of the mixing system geometry and of a fed-batch strategy to increase glucose concentration. Renewable Energy, Elsevier, 2019, 131, pp.152-158. ⟨10.1016/j.renene.2018.07.038⟩. ⟨hal-02063461⟩

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