Genome and transcriptome of the natural isopropanol producer Clostridium beijerinckii DSM6423

Frederique Bidard (1) , Angélique Bisson (1) , Benjamin Clément (1) , Etienne Jourdier (1) , Ana María López-Contreras (2) , Nicolas Lopes Ferreira (1) , Hadrien Máté de Gérando (2, 1) , François Wasels (1)

1 IFPEN - IFP Energies nouvelles
2 Wageningen Food & Biobased Research

There is a worldwide interest for sustainable and environmentally-friendly ways to produce fuels and chemicals from renewable resources. Among them, the production of acetone, butanol and ethanol (ABE) or Isopropanol, Butanol and Ethanol (IBE) by anaerobic fermentation has already a long industrial history. Isopropanol has recently received a specific interest and the best studied natural isopropanol producer is C. beijerinckii DSM 6423 (NRRL B-593). This strain metabolizes sugars into a mix of IBE with only low concentrations of ethanol produced (< 1 g/L). However, despite its relative ancient discovery, few genomic details have been described for this strain. Research efforts including omics and genetic engineering approaches are therefore needed to enable the use of C. beijerinckii as a microbial cell factory for production of isopropanol.

Keywords: Clostridium beijerinckii DSM6423 Genome RNA-seq transcriptome Clustering IBE fermentation Isopropanol

Domains: Life Sciences [q-bio]; Development Biology; Humanities and Social Sciences; Gender studies

Frederique Bidard, Angélique Bisson, Benjamin Clément, Etienne Jourdier, Ana María López-Contreras, et al.. Genome and transcriptome of the natural isopropanol producer Clostridium beijerinckii DSM6423. BMC Genomics, 2018, 19 (1), ⟨10.1186/s12864-018-4636-7⟩. ⟨hal-01884563⟩