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Structural evidence for a [4Fe-5S] intermediate in the non-redox desulfuration of thiouracil
Abstract : We recently discovered a [Fe-S]-containing protein with in vivo thiouracil desulfidase activity called TudS. We report here the crystal structure of TudS, refined at 1.5 Å resolution, which harbors a [4Fe-4S] cluster, bound by three cysteines only. Incubation of TudS crystals with 4-thiouracil trapped the cluster with a hydrosulfide ligand bound to the fourth non-protein-bonded iron, as established by the sulfur anomalous signal. This indicates that a [4Fe-5S] state of the cluster is a catalytic intermediate in the desulfuration reaction. Structural data and site-directed mutagenesis indicate that a water molecule is located next to the hydrosulfide ligand and to two catalytically important residues, Ser101 and Glu45. This information together with modeling studies allow us to propose a mechanism for the unprecedented nonredox enzymatic desulfuration of thiouracil, in which a [4Fe-4S] cluster binds and activates the sulfur atom of the substrate.
Cited literature [50 references]
https://hal.archives-ouvertes.fr/hal-02943904
Contributor : Béatrice Golinelli-Pimpaneau <>
Submitted on : Monday, September 21, 2020 - 8:03:31 AM
Last modification on : Thursday, January 7, 2021 - 4:36:05 PM
Long-term archiving on: : Thursday, December 3, 2020 - 2:20:45 PM
Contributor : Béatrice Golinelli-Pimpaneau <>
Submitted on : Monday, September 21, 2020 - 8:03:31 AM
Last modification on : Thursday, January 7, 2021 - 4:36:05 PM
Long-term archiving on: : Thursday, December 3, 2020 - 2:20:45 PM
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