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Stereospecific Isotopic Labeling of Methyl Groups for NMR Spectroscopic Studies of High-Molecular-Weight Proteins

Abstract : Sometimes less is more: [13C1H3]methyl isotopomers can be biosynthetically incorporated specifically into the pro-S methyl groups of leucine and valine residues in large protein assemblies within a perdeuterated background by using an acetolactate precursor. This stereospecific labeling strategy considerably enhances NMR spectra for large protein assemblies.
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https://hal.archives-ouvertes.fr/hal-01143348
Contributor : Nathalie Gon <>
Submitted on : Tuesday, June 22, 2021 - 8:49:08 AM
Last modification on : Tuesday, July 27, 2021 - 10:28:03 AM

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Pierre Gans, Olivier Hamelin, Rémy Sounier, Isabel Ayala, M Asunción Durá, et al.. Stereospecific Isotopic Labeling of Methyl Groups for NMR Spectroscopic Studies of High-Molecular-Weight Proteins. Angewandte Chemie International Edition, Wiley-VCH Verlag, 2010, 49 (11), pp.1958-1962. ⟨10.1002/anie.200905660⟩. ⟨hal-01143348⟩

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