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A simple biosynthetic method for stereospecific resonance assignment of prochiral methyl groups in proteins

Abstract : A new method for stereospecific assignment of prochiral methyl groups in proteins is presented in which protein samples are produced using U-[13C]glucose and subsaturating amounts of 2-[13C]methyl-acetolactate. The resulting non-uniform labeling pattern allows proR and proS methyl groups to be easily distinguished by their different phases in a constant-time two-dimensional 1H-13C correlation spectra. Protein samples are conveniently prepared using the same media composition as the main uniformly-labeled sample and contain higher levels of isotope-enrichment than fractional labeling approaches. This new strategy thus represents an economically-attractive, robust alternative for obtaining isotopically-encoded stereospecific NMR assignments of prochiral methyl groups.
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https://hal.archives-ouvertes.fr/hal-01138581
Contributor : Nathalie Gon <>
Submitted on : Monday, June 21, 2021 - 10:38:06 AM
Last modification on : Tuesday, July 27, 2021 - 10:28:03 AM

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Michael J Plevin, Olivier Hamelin, Jérôme Boisbouvier, Pierre Gans. A simple biosynthetic method for stereospecific resonance assignment of prochiral methyl groups in proteins. Journal of Biomolecular NMR, Springer Verlag, 2011, 49 (2), pp.61-67. ⟨10.1007/s10858-010-9463-3⟩. ⟨hal-01138581⟩

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