In Vitro Reconstitution of Dynein Force Exertion in a Bulk Viscous Medium

Héliciane Palenzuela; Benjamin Lacroix; Jérémy Sallé; Katsuhiko Minami; Tomohiro Shima; Antoine Jegou; Guillaume Romet-Lemonne; Nicolas Minc

doi:10.1016/j.cub.2020.08.078

Journal articles

In Vitro Reconstitution of Dynein Force Exertion in a Bulk Viscous Medium

Héliciane Palenzuela ¹ Benjamin Lacroix ¹ Jérémy Sallé ¹ Katsuhiko Minami ² Tomohiro Shima ² Antoine Jegou ¹ Guillaume Romet-Lemonne ^{1, *} Nicolas Minc ^{1, *}

* Corresponding author

1 IJM (UMR_7592) - Institut Jacques Monod

2 Graduate School of Science, The University of Tokyo, 113-0033 Tokyo, Japan

Abstract : Highlights d A bulk motility assay reconstitutes dynein pulling microtubules in the cytoplasm d A single motile cargo in a viscous solution generates near pN forces on a microtubule d Forces are additive, recapitulating a length-dependent model d Tug-of-war situations yield centration of minimal asters in bulk

Document type :

Journal articles

Domain :

Life Sciences [q-bio]

Life Sciences [q-bio] / Biochemistry, Molecular Biology / Biophysics

Life Sciences [q-bio] / Cellular Biology

Life Sciences [q-bio] / Biochemistry, Molecular Biology

Life Sciences [q-bio] / Cellular Biology / Subcellular Processes [q-bio.SC]

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https://hal.archives-ouvertes.fr/hal-03025707
Contributor : Benjamin Lacroix <>
Submitted on : Thursday, November 26, 2020 - 3:53:40 PM
Last modification on : Monday, December 28, 2020 - 10:56:05 AM

Palenzuela_MS_R2.pdf

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